Site-specific integration of the conjugal Vibrio cholerae SXT element into prfC

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Vibrio cholerae SXT Element, Laos

To the Editor: The SXT element is a Vibrio cholerae–derived ICE (integrating and conjugative element), which has also been referred to as a conjugative transposon (1) or a constin (2). ICEs excise from the chromosomes of their hosts, transfer to a new host through conjugation, and then integrate into the chromosome again. SXT element was originally isolated in 1993 from a V. cholerae O139 clini...

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Control of SXT integration and excision.

The Vibrio cholerae SXT element is a conjugative self-transmissible chromosomally integrating element that encodes resistance to multiple antibiotics. SXT integrates in a site-specific fashion at prfC and excises from the chromosome to form a circular but nonreplicative extrachromosomal form. Both chromosomal integration and excision depend on an SXT-encoded recombinase, Int. Here we found that...

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Vibrio cholerae O1 from Accra, Ghana carrying a class 2 integron and the SXT element

OBJECTIVES Vibrio cholerae O1 from a 2006 outbreak in Accra were commonly resistant to multiple antimicrobials and, in particular, to trimethoprim/sulfamethoxazole, drugs commonly used in the treatment of cholera. We sought to determine the genetic basis for trimethoprim/sulfamethoxazole resistance in outbreak isolates. METHODS Twenty-seven isolates from the outbreak were screened by PCR and ...

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Mechanistic insights into filamentous phage integration in Vibrio cholerae

Vibrio cholerae, the etiological agent of acute diarrhoeal disease cholera, harbors large numbers of lysogenic filamentous phages, contribute significantly to the host pathogenesis and provide fitness factors to the pathogen that help the bacterium to survive in natural environment. Most of the vibriophage genomes are not equipped with integrase and thus exploit two host-encoded tyrosine recomb...

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Mobilization of plasmids and chromosomal DNA mediated by the SXT element, a constin found in Vibrio cholerae O139.

The Vibrio cholerae SXT element encodes resistance to multiple antibiotics and is a conjugative, self-transmissible, and chromosomally integrating element (a constin). Excision and self-transfer of the SXT element require an element-encoded integrase. We now report that the SXT element can also mobilize the plasmids RSF1010 and CloDF13 in trans as well as chromosomal DNA in an Hfr-like manner. ...

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ژورنال

عنوان ژورنال: Molecular Microbiology

سال: 1999

ISSN: 0950-382X,1365-2958

DOI: 10.1046/j.1365-2958.1999.01330.x